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Evaluation of the atzB gene as a functional marker for the simazine-degrading potential of an agricultural soil

机译:atzB基因作为功能性标记对农业土壤中simazine降解潜力的评估

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摘要

The occurrence of natural simazine-degrading bacteria could be an important limiting factor to the use of the herbicide in those agricultural soils with a wide history of herbicide applications. In this work the potential of an agricultural soil to degrade simazine and the effect of the addition of urea was assayed in both fertilised and unfertilised soil microcosms. A culture-independent approach based on the fluorescence in situ hybridisation (FISH) technique, using a specific oligonucleotide probe (AtzB1), was applied to detect simazine-degrading bacteria in the soil microcosms. The presence of the atzABC genes in the agricultural soil was confirmed by PCR from soil-extracted DNA. The percentage of AtzB1 probe-target cells in the urea-untreated soil was higher than in the urea-treated one. Moreover, the greatest percentage of AtzB1 probe-target cells in the urea-untreated soil was accompanied by a greater degradation rate, compared to the urea-fertilised one. Our results indicate that the proposed approach was sensitive enough to detect changes in the natural simazine degradation capacity of the soil after fertilisation practices, which typically involve a nitrogen increase
机译:在具有广泛除草剂应用历史的那些农业土壤中,天然可降解Simazine的细菌的出现可能是重要限制因素。在这项工作中,在施肥和未施肥的土壤微观世界中都分析了农业土壤降解西马津的潜力和添加尿素的影响。基于荧光原位杂交(FISH)技术的非培养独立方法,使用特定的寡核苷酸探针(AtzB1),用于检测土壤微观世界中降解了simazine的细菌。通过土壤提取DNA的PCR证实了农业土壤中atzABC基因的存在。未经尿素处理的土壤中AtzB1探针靶细胞的百分比高于经过尿素处理的土壤中的百分比。此外,与未施尿素的土壤相比,未施尿素的土壤中最大百分比的AtzB1探针-靶细胞伴随着更高的降解率。我们的结果表明,所提出的方法足够灵敏,可以检测施肥后土壤中自然西马津嗪降解能力的变化,这通常涉及氮的增加

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