利用 GS3基因功能性分子标记改良水稻粒型的研究

摘要

以长粒品种‘三粒寸’‘IAC1246’‘JAPPENI TUNGKUNGO’和‘MIGA’作为供体亲本，以优良选系SAGC-4作为受体亲本，进行杂交和连续回交，利用 GS3基因的功能性分子标记 SF28进行 BC1 F1和 BC2 F1的分子标记辅助选择。在不同 BC2 F1单株派生的 BC2 F2分离群体中，观察到粒长性状的典型双峰分布或单峰连续分布。结合田间农艺性状考察，选出89个具备细长粒型且综合农艺性状良好的单株，其中4个优良单株的平均粒长从原始受体亲本的8．32 mm 增加至9．84 mm 左右，平均长宽比由原来的2．61增加至3．00。结果表明：GS3基因具有控制粒长和长宽比的较大遗传效应，对该基因进行分子标记辅助选择可快速改良亲本的粒型性状。%Four donor parental lines with long grain shape,‘SANLICUN ’,‘IAC1 246 ’,‘JAPPENI TUNGKUNGO’and ‘MIGA’,were crossed with a promising indica rice line SAGC-4 as the recipient parent,fol-lowed by continuous backcrosses.Marker-assisted selection was conducted in both BC1 F1 and BC2 F1 generations by using the CAPs marker SF28 of GS3 gene.Segregation of grain length showed typical bimodal or continuous distributions in BC2 F2 populations.A set of 89 individuals were selected based on both grain shape and visual in-spection on other agronomic traits.Among them,4 promising individuals had average grain length of 9.84 mm and length?width ratio of 3.00,in comparison to 8.32 mm and 2.61 of the original recipient parent,respectively. The results indicated that GS3 gene had large genetic effects on both grain length and length?width ratio.Marker-assisted selection of the gene could accelerate the improvement of rice grain shape.