Development of an in vitro method for determination of methane production kinetics using a fully automated in vitro gas system - a modelling approach.

摘要

The objective of the present study was to estimate methane production using the kinetic parameters from an automated in vitro gas production (GP) system in a mechanistic, dynamic rumen model. Four sample sizes [300 mg (5 g/l), 600 mg (10 g/l), 900 mg (15 g/l) and 1200 mg (20 g/l)] of timothy hay were incubated in 60 ml buffered rumen fluid in an automated in vitro system to determine methane and total gas production. A logarithmic model (R2