A method for the determination of pharmacokinetics or toxicological concerns kinetics of test substances with the aid of in - vitro - cell culture systems and devices suitable therefor

摘要

the invention concerns a method for the determination of pharmacokinetics. toxicokinetics of testsubstanzen ex vivo. it is erfindungsgemu00e4u00df for testing an in vitro cell culture in blasenfrei begasten wirbelschichtreaktor used. moreover, in tru00e4gerku00f6rpern cells of 2 million, the proportion relative to the entire response system on 1%.furthermore, the invention relates to a device for the cultivation of human or animal organfunktionszellen, especially for carrying out pharmacokinetic or toxikokinetiktests in vitro.erfindungsgemu00e4u00df, that is the device to a wirbelschichtreaktor with integrated membranbegasung with porous tru00e4gerku00f6rpern of 1000 m in diameter, porengru00f6u00dfen 100 m and a porosity of 50% to the immobilization of the cells.the other is the workload, including rezyklierungskreis 100 ml with a schu00fcttvolumenanteil the tru00e4gerku00f6rper, relative to the volume of work, from 0.2 to 0.6.