Voltammetric determination of butylated hydroxyanisole,butylated hydroxytoluene,propyl gallate and tert-butylhydroquinone by use of chemometric approaches

摘要

The voltammetric behavior of synthetic food antioxidants,such as butylated hydroxyanisole (BHA),butylated hydroxytoluene(BHT),propyl gallate (PG)and tert-butylhydroquinone(TBHQ),at a glassy carbon electrode in dolution of 0.1 moll~(-1) perchloric acid containing 1% methanol has beeninvestigated. The obtained linear sweep voltammograms (LSV)showed that allthese four compounds have well-defined oxidation waves with peak potentials of 629,818,599and 501 mV for BHA,BHT,PG and TBHQ,respectively.Linear calibration graphs were also obtained in the concentration ranges of 0.5-15.0,0.5-0.8,1.0-15.0 and 1.0-15.0 mgl~(-1) for BHA,BHT,PG and TBHQ,respectively.However,the voltammetric peaks of these antioxidants seriously overlap and it is difficult to determine them individually from a mixture without prior separation.In this work,a method for simultaneous determination of these antioxidants,based on their oxidation at the glassy carbon electrode,with the aid of chemometric approaches,such as classical least wquares (CLS),principal component regression (PCR) and partial least squares (PLS),has been developed.The method was applied to determine the four antiodants in a set of synthetic mixtures and several commercial food samples;in general,satisfactory results were obtained.