Effects of Butylated Hydroxyanisole on the Metabolism and Mutagenic and Transformation Potentials of Benzo(A)-Pyrene in Cultured Mammalian Cells

摘要

The mechanism by which butylated hydroxyanisole (BHA) inhibits benzo(a)pyrene (BaP)-induced neoplasia in vivo and mutagenicity in vitro has been the subject of a number of investigations. In this study the effect of BHA on BaP metabolism in Syrian hamster embryonic (SHE) cells was studied. In addition, a SHE cell-mediated Chinese hamster ovary (CHO) cell mutagenesis and a SHE cell transformation assay have been used to study the biological effects of BHA. The results indicate that BHA reduces the metabolism of BaP in SHE cells, suppresses BaP-induced mutagenicity in target CHO cells and reduces morphological transformation by BaP in SHE cells. (ERA citation 08:010785)