Prediction of an antisense-effective target site on VEGFmRNA by using RNase H and a library of random oligonucleotides

摘要

End(5' or 3')-P-32 -labelled mRNA coding VEGF (vascular endothelial growth factor) was cleaved by RNase H in the presence of a library of oligodeoxyribonucleotides with random sequences of 20-mer. The most susceptible site, determined by gel electrophoresis and autoradiography, agreed with the most effective antisense target site found with cell-free experiments. Based on these results, the method presented here is :indicated to be useful to predict an antisense-effective target site on mRNA. (C) 1998 Elsevier Science B.V. [References: 9]