Quantitative Lateral Flow Immunoassay for Total Prostate Specific Antigen in Serum

摘要

A simple method for the rapid determination of prostate-specific antigen (PSA) in serum is reported using a lateral flow immunoassay with gold nanoparticles as the label. The method uses the intensity of colored test lines to determine PSA from 0.3 to 30ng/mL. The limit of detection was 0.3ng/mL and the coefficient of variation was less than 10%. The analysis time was approximately 20min. The novel method showed good correlation with enzyme linked immunosorbent assay (ELISA) measurements of prostate-specific antigen concentration in human serum with a linear regression coefficient of 0.985. The developed system was stable for at least 12 months when stored from +4 to 30 degrees C and has potential application for clinical practice.