Kinetic Spectrophotometric Determination of Thiols and Ascorbic Acid

摘要

This paper describes a kinetic spectrophotometric method for the determination of L-ascorbic acid(AA)and thiols(RSH).Absorbance of Fe(II)-phen complex formed during the reaction of AA or RSH with Fe(III)-phen was continuously measured at 510 nm by double-beam spectrophotometer with flow cell.For determination some thiols,the catalytic effect of Cu~(2+)ions was used.AA and RSH can be determined in concentration ranges from 4.0 x 10~(-6)to 4.0 x 10~(-5)M and from 8.0 x 10~(-6)to 8.0 x 10~(-5)M,respectively.The applicability of the proposed method was demonstrated by determination of chosen compounds in pharmaceutical dosage forms.