Quantification of Levosulpiride in Human Plasma by High-Performance Liquid Chromatography

摘要

An analytical procedure was developed and validated for the quantification of levosulpiride in human plasma.After subjecting a plasma sample to a two-step extraction procedure,an aliquot of the aqueous phase was injected onto an high-performance liquid chromatography system equipped with a fluorescence detector.The detector response was linear for levosulpiride concentrations in the range of 2.5 to 500 ng/ml.The intra- and inter-day precision was below 15.4 and 10.1%,and the accuracy was in a range from 89.7 to 109.4%.The method is applicable for use in the pharmacokinetic characterization of levosulpiride after a 75-mg oral dose in humans.