Objective: To establish a high-performance liquid chromatographic (HPLC) method for the concentration determination of levetiracetam in human plasma. Methods: With metronidazole as the internal standard, acetonitrile was added to plasma sample for precipitating protein. The supernatant was washed with dichloromethane, and the upper aqueous was injected to HPLC. Hypersil BDS column (4.6 mm × 250 mm, 5 μm) was selected. And the mobile phase was a mixture of water and acetonitrile (87 : 13, v/v) at a flow rate of 1.0 mL·min-1. The detection wavelength was 205 nm. Column temperature was 40 ℃ and injection volume was 20 μL. Results: Levetiracetam showed a good linear relation in the range of 2.5 – 80.0 μg·mL-1with a good correlation coefficient (r = 0.999?4). The intra-batch variations were ranged from 1.06% to 1.97% and inter-batch variations were ranged from 3.46% to 8.14%. The mean recoveries were ranged from 94.00% to 102.48%. Conclusion: The method was sensitive, specific and simple. It is suitable for monitoring the concentration of levetiracetam in human plasma.%目的:建立人血浆中左乙拉西坦(LEV)的高效液相色谱测定方法.方法:以甲硝唑为内标,血浆样品经乙腈沉淀蛋白,二氯甲烷提取去杂质,取水相进样分析.采用Hypersil BDS色谱柱(4.6 mm × 250 mm,5 μm),流动相为水-乙腈(87 : 13, v/v),流速1.0 mL·min-1,检测波长205 nm,柱温40℃,进样量20μL.结果:LEV在2.5～80.0μg·mL-1范围内线性关系良好(r = 0.999 4),批内变异(RSD)在1.06% ～ 1.97%之间,批间变异(RSD)在3.46% ～ 8.14%之间,方法学回收率为94.00% ～102.48%.结论:本方法灵敏度高,操作简便易行,可用于临床LEV血药浓度的监测.
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