Hyperoxia-mediated transcriptional activation of cytochrome P4501A1 ( CYP1A1 ) and decreased susceptibility to oxygen-mediated lung injury in newborn mice

摘要

Abstract Hyperoxia contributes to the development of bronchopulmonary dysplasia (BPD) in premature infants. In this study, we tested the hypothesis that newborn transgenic mice carrying the human CYP1A1- Luc promoter will display transcriptional activation of the human CYP1A1 promoter in?vivo upon exposure to hyperoxia, and that these mice will be less susceptible to hyperoxic lung injury and alveolar simplification than similarly exposed wild type (WT) mice. Newborn WT (CD-1) or transgenic mice carrying a 13.2?kb human CYP1A1 promoter and the luciferase (Luc) reporter gene ( CYP1A1 -luc) were maintained in room air or exposed to hyperoxia (85% O 2 ) for 7–14 days. Hyperoxia exposure of CYP1A1- Luc mice for 7 and 14 days resulted in 4- and 30-fold increases, respectively, in hepatic Luc ( CYP1A1 ) expression, compared to room air controls. In lung, hyperoxia caused a 2-fold induction of reporter Luc at 7 days, but the induction declined after 14 days. The newborn CYP1A1- Luc mice were less susceptible to lung injury and alveolar simplification than similarly exposed wild type (WT) CD-1 mice. Also, the CYP1A1 -Luc mice showed increased levels of hepatic and pulmonary CYP1A1 expression and hepatic CYP1A2 activity after hyperoxia exposure. Hyperoxia also increased NADP(H) quinone reductase (NQO1) pulmonary gene expression in both CD-1 and CYP1A1 -Luc mice at both time points, but this was more pronounced in the latter at 14 days. Our results support the hypothesis that hyperoxia activates the human CYP1A1 promoter in newborn mice, and that increased endogenous expression of CYP1A1 and NADP(H) quinone reductase (NQO1) contributes to the decreased susceptibilities to hyperoxic lung injury in the transgenic animals. This is the first report providing evidence of hyperoxia-mediated transcriptional activation of the human CYP1A1 promoter in newborn mice, and this in conjunction with decreased lung injury, suggests that these phenomena have important implications for BPD. Highlights ? Hyperoxia causes enhanced transcriptional activation of human CYP1A1-Luciferase expression in newborn mice in?vivo . ? Transgenic mice carrying the human CYP1A1-luc promoter are less susceptible to hyperoxic lung in injury and in?vivo . ? CYP1A1 -Luc mice display increased levels of endogenous hepatic and pulmonary CYP1A as well as NADP(H) quinone oxidoreductase (NQO1) expression after hyperoxia exposure, compared to wild type mice. ? The increased endogenous expression of CYP1A1 and NQO1 contributes to the beneficial effects seens in CYP1A1-Luc mice.