The effect of exogenous GSH, GSSG and GST-E on glutathione concentration and activity of selected glutathione enzymes in the liver, kidney and muscle of mice

摘要

The experiment was conducted on randomly chosen 6 weeks-old mice, divided into four groups of10 animals. The animals of control group were intraperitoneally injected with 250 jjl of 0.9% NaClwhereas those of three experimental groups with reduced glutathione (GSH), oxidized glutathione(GSSG) and glutathione ethyl ester (GST-E) at doses 250 fig/kg body weight. Glutathioneconcentration as well as activity of glutathione enzymes was determined in liver, kidney and muscletissues of control and experimental mice. Injections of GSH led to significant increase in the concentration of reduced glutathione in the liver only, increased activity of glutathione transferase and glutathione peroxidase in the liver, kidney and muscle, and of glutathione reductase in liver and kidney. Injections of GSSG caused the significant decrease in GSH concentration of the kidney while theactivity of glutathione transferase rised in all three organs. The glutathione peroxidase rised onlyin the muscle. Injections of GST-E increased the glutathione concentration in the liver and muscle, and glutathione transferase activity in all three tissues. Simultaneously, the activity of glutathione peroxidase in both liver and kidney as well as of glutathione reductase in the muscle dropped.