Detection of L-alanylaminopeptidase activity in microorganisms using chromogenic self-immolative enzyme substrates

Cellier Marie; James Arthur L.; Orenga Sylvain; Perry John D.; Rasul Ari K.; Stanforth Stephen P.

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Detection of L-alanylaminopeptidase activity in microorganisms using chromogenic self-immolative enzyme substrates

机译：使用发色自焚酶基材检测微生物中的L-醛酰胺肽酶活性

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摘要

Three potential chromogenic enzymatic probes, each possessing a self-immolative spacer unit, were synthesised for the purpose of detecting L-alanylaminopeptidase activity in microorganisms. An Alizarin based probe was the most effective, allowing several species to generate strongly coloured colonies in the presence of metal ions. (C) 2017 Elsevier Ltd. All rights reserved.

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来源
《Bioorganic and Medicinal Chemistry Letters》 |2017年第10期|共5页
作者
Cellier Marie; James Arthur L.; Orenga Sylvain; Perry John D.; Rasul Ari K.; Stanforth Stephen P.;
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作者单位

BioMerieux SA Res &

Dev Microbiol 3 Route Port Michaud F-38390 La Balme Les Grottes France;

Northumbria Univ Dept Appl Sci Newcastle Upon Tyne NE1 8ST Tyne &

Wear England;

BioMerieux SA Res &

Dev Microbiol 3 Route Port Michaud F-38390 La Balme Les Grottes France;

Freeman Rd Hosp Dept Microbiol Newcastle Upon Tyne NE7 7DN Tyne &

Wear England;

Northumbria Univ Dept Appl Sci Newcastle Upon Tyne NE1 8ST Tyne &

Wear England;

Northumbria Univ Dept Appl Sci Newcastle Upon Tyne NE1 8ST Tyne &

Wear England;

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原文格式 PDF
正文语种 eng
中图分类药学;
关键词
Chromogenic enzyme substrates; L-Alanylaminopeptidase detection; Microorganisms;

机译：发色酶酶;L-醛酰胺肽酶检测;微生物;

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专利

1. Detection of L-alanylaminopeptidase activity in microorganisms using chromogenic self-immolative enzyme substrates [J] . Cellier Marie, James Arthur L., Orenga Sylvain, Bioorganic and Medicinal Chemistry Letters . 2017,第10期

机译：使用发色自焚酶基材检测微生物中的L-醛酰胺肽酶活性
2. Novel chromogenic aminopeptidase substrates for the detection and identification of clinically important microorganisms [J] . Cellier Marie, James Arthur L., Orenga Sylvain, Bioorganic and medicinal chemistry . 2014,第19期

机译：用于检测和鉴定临床重要微生物的新型发色氨基肽酶底物
3. Synthesis and evaluation of novel chromogenic aminopeptidase substrates for microorganism detection and identification. [J] . James AL, Perry JD, Rigby A, Bioorganic and Medicinal Chemistry Letters . 2007,第5期

机译：用于微生物检测和鉴定的新型生色氨基肽酶底物的合成和评估。
4. Activities of microorganisms and enzymes in water-restricted environments: biological activities in aqueous compartments at micron scale [C] . Michael Hoppert, Klaus Mlejnek, Beatrix Seiffert, Society of Photo-Optical Instrumentation Engineers Conference on Instruments, Methods, and Missions for the Investigation of Extraterrestrial Microorganisms . 1997

机译：水限制性环境中微生物和酶的活动：微米尺度水隔间的生物活性
5. A novel fiber-optic probe for detection of enzyme activity using conventional substrates. [D] . Sheng, Feng. 2005

机译：一种使用常规底物检测酶活性的新型光纤探针。
6. Synthesis and Evaluation of Chromogenic and Fluorogenic Substrates for High-ThroughputDetection of Enzymes That Hydrolyze Inorganic Polyphosphate [O] . CarleighF. F. Hebbard, Yan Wang, Catherine J. Baker, -1

机译：高通量发色和荧光底物的合成与评价水解无机多磷酸盐的酶的检测
7. Detection of l-alanylaminopeptidase activity in microorganisms using fluorogenic self-immolative enzyme substrates [O] . Cellier Marie, James Arthur, Lowe Jonathan, 2016

机译：使用荧光自消旋酶底物检测微生物中的l-丙氨酰氨基肽酶活性
8. U-3'-BCIP: A Chromogenic Substrate for the Detection of RNase A in RecombinantDNA Expression Systems [R] . Witmer, M. R., Falcomer, C. M., Weiner, M. P., 1991

机译：U-3'-BCIp：用于检测重组DNa表达系统中RNase a的显色底物

Detection of L-alanylaminopeptidase activity in microorganisms using chromogenic self-immolative enzyme substrates

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