Detection of l-alanylaminopeptidase activity in microorganisms using fluorogenic self-immolative enzyme substrates

摘要

A series of fluorogenic enzymatic substrates that incorporate a self-immolative spacer were synthesised for the purpose of identifying l-alanylaminopeptidase activity in microorganisms in agar media. These substrates resulted in the generation of fluorescent microorganism colonies with Gram-negative microorganisms.