Expression of rice glutamate decarboxylase in Bifidobacterium longum enhances y-aminobutyric acid production

摘要

Bifidobacteria are important for the production of fermented dairy products and probiotic formulas but have a low capacity for gamma-aminobutyric acid (GABA) production.To develop a Bifidobacterium strain with an enhanced GABA production,we transformed Bifidobacterium longum with a rice glutamate decarboxylase (OsGADC~-) gene by electroporation.When the transformed strain was cultured in medium containing monosodium glutamate,the amount of GABA increased significantly compared with those of untransformed Bifidobacterium.Thus,by introducing a plant derived GAD gene,a Bifidobacterium strain has been genetically engineered to produce high levels of GABA from glutamate.