Expression of Bacillus macerans cyclodextrin glucanotransferase gene in Saccharomyces cerevisiae

摘要

Cyclodextrin glucanotransferase (CGTase) gene of Bacillus macerans was subcloned down-stream of yeast ADH1 promoter and expressed in Saccharomyces cerevisiae.Most of the CGTase expressed was in the extracellular medium with a maximum activity of about 0.28 unit ml~(-1) after 48 h cultivation.The recombinant CGTase was secreted as an N-linked-glycosylated form and predominantly produced #alpha#-cyclodextrin from starch.