Production of a fusion protein of sweet potato sporamin from recombinant E.coli XL1 Blue by fed-batch fermentations

摘要

Glucose-stat and pH-stat control strategies were employed in order to culture a recombinant E.coli XL1 Blue to produce a fusion protein of sweet potato sporamin (SPA) and glutathione S-transferase (GST) from the recombinant E.coli XL1 Blue.Cell densities up to 25 g 1~(-1) and 28.9 mg fusion protein (GST-SPA)g~(-1) cell dry weight (CDW) was achieved from a fed-batch fermentation controlled by glucose-stat strategy.A pH-stat control fermentation using glycerol as a carbon source gave E.coil up to 27 g 1~(-1) and 31.5 mg GST-SPA g~(-1) CDW.Additionally,a pH-stat control strategy using glucose as a carbon source gave E.coli up to 15 g 1~(-1) and about 22.7 mg g~(-1) CDW of GST-SPA.