The property of active substance from royal jelly in CRE-mediated transcriptional activity

摘要

To determine the active substances of royal jelly (RJ), we fractionated RJ to protein, peptide and low molecular fraction and examined the effects of RJ fraction on CRE-mediated transcription and protein phosphorylation. Only low molecular substance of RJ increased CRE-mediated transcription in PCI 2D cells. Moreover, this fraction induced CREB and ERK phosphorylation. These results suggest that the activities of RJ on CRE-mediated transcription and protein phosphorylation depend on low molecular active substance. Moreover, these activities are resistant to protease. RJ may activate effectively neuronal functions via enhancement of CRE-mediated transcription.