An Interview With…

摘要

The development of the endpoint chromogenic technology was a critical step in the development of the kinetic chromogenic assays that are in regular use today around the world. The discovery was based on determining the sequence of amino acids at the cleavage site of coagulin, the last step in the enzymatic cascade leading to gel clot formulation in the classical gel-clot LAL assay. Once this sequence was known, a peptide could be sequenced that couid be attached to a chromophore. When the chromophore was cleaved from the peptide, a yellow color was generated. The amount of color generated by enzymatic cleavage was proportionate to the amount of endotoxin in the sample, and could be measured using a spectrophotometer. Generation of color development, rather than a clot or turbidity, made it easier to test samples that were not colorless.