Cloning, sequencing and heterologous expression of a new chitinase gene, chi92, from Streptomyces olivaceoviridis ATCC 11238

摘要

A new chitinase gene, chi92, encoding the largest known chitinase from Streptomyces olivaceoviridis ATCC 11238 was sequenced by means of different PCR-methods. The cloned gene was expressed in E. coli and the recombinant protein could be detected by Western-blot analysis. The multiplicity of chitinolytic enzymes of this strain is discussed. [References: 31]