DNA FRAGMENT PREPARED BY MOLECULAR CLONING FROM STREPTOMYCES GLISEUS ATCC 10137 ENCODING SAF-POLYPEPTIDE, SAF-POLYPEPTIDE, RECOMBINANT PLASMID DNA DETERMINING EXPRESSION OF SAF-POLYPEPTIDE (VARIANTS), RECOMBINANT PLASMID DNA PULAD3, RECOMBINANT PLASMID DNA PULAD300, STRAIN OF FUNGUS STREPTOMYCES LIVIDANS CONTAINING RECOMBINANT PLASMID DNA PULAD3 AND A METHOD OF SAF-POLYPEPTIDE EXPRESSION

摘要

FIELD: molecular biology, genetic engineering. SUBSTANCE: invention relates to DNA fragment encoding SAF-polypeptide that is an activation factor of the secondary metabolism. Fragment is prepared by method of molecular cloning from Streptomyces griseus cells ATCC 10137. This fragment is a component of recombinant plasmid DNAs providing expression of SAF-polypeptide. Also, this fragment is used for transformation of microorganism cells which are cultured then on nutrient medium and expression of SAF-polypeptide is detected. One of above indicated plasmid is a component of the strain Streptomyces lividans. This strain shows traits that are typical for Streptomycetes. EFFECT: DNA fragment indicated above, improved method of preparing. 8 cl, 13 dwg