Cloning of the biosynthetic pathway genes for chlortetracycline production from streptomyces aureofaciens and their expression in streptomyces lividans

摘要

The present invention relates to the cloning, isolation and DNA (isolated fragment from Streptomyces aureofaciens) which directs the synthesis of tetracycline and chlortetracycline, in a heterologous host such as Streptomyces lividans. The claimed plasmids are isolated by screening a cosmid library, constructed in Escherichia coli, for the expression of tetracycline resistance in S. lividans.