LncRNA PTCSC3/miR‐574‐5p Governs Cell Proliferation and Migration of Papillary Thyroid Carcinoma via Wnt/β‐Catenin Signaling

摘要

ABSTRACT The distance metastases of papillary thyroid carcinoma (PTC) were a major threaten for PTC patients, thus, to study the potential mechanism for the treatment of PTC was essential. Previous studies have shown that PTCSC3 (Thyroid Carcinoma Susceptibility Candidate 3), miR‐574‐5p and Wnt/β‐catenin were involved in PTC, but the potential pathogenic mechanism among them was still unclear. Real‐time PCR and Western blot were used to detect genes expression. Luciferase reporter assay was used to detect the combination of miR‐574‐5p and suppressor of cancer cell invasion (SCAI), as well as the ratio of TOP/FOP. RNA Pull‐down assay verified the bound of PTCSC3 and miR‐574‐5p. MTT assay, Transwell assay, and wound scratch assay were used to detect cell viability and cell migration. The expression of PTCSC3 and SCAI were decreased, while miR‐574‐5p and β‐catenin were increased in PTC tissues and cells. Overexpressed PTCSC3 suppressed cell proliferation and migration, promoted the expression of SCAI, but inhibited β‐catenin. PTCSC3 absorbed miR‐574‐5p, and miR‐574‐5p targeted to SCAI; SCAI could regulate the activity of Wnt/β‐catenin. PTCSC3/miR‐574‐5p regulated the activity of Wnt/β‐catenin via SCAI and mediated cell proliferation and migration of PTC‐1. In vivo experiments verified the fact that overexpressed PTCSC3 inhibited tumor growth. The signaling PTCSC3‐miR‐574‐5p‐SCAI‐Wnt/β‐catenin mediated the proliferation and migration of PTC‐1 cells, which was vital for the further PTC therapy and prognosis. J. Cell. Biochem. 118: 4745–4752, 2017. ? 2017 Wiley Periodicals, Inc.