Simultaneous determination of pyrametostrobin and its two metabolites in cucumber and soil using a quick, easy, cheap, effective, rugged, and safe method with HPLC-MS/MS

摘要

An easy, effective and sensitive analytical method for the simultaneous determination of a novel fungicide pyrametostrobin and its two metabolites pyrametostrobin-M1 and pyrametostrobin-M2 in cucumber and soil was developed using a quick, easy, cheap, effective, rugged, and safe method with high-performance liquid chromatography and tandem mass spectrometry. The extraction solvent was acetonitrile, and cleanup sorbents were primary secondary amine and graphitized carbon black for cucumber samples and primary secondary amine for soil samples. The three target compounds were successfully separated between 3.2 and 3.9 min using aWaters CORTECS (TM) C-18 column connected to an electrospray ionization source. All the matrix-matched samples at three fortified levels (10, 100 and 1000 mu g/kg) provided satisfactory recoveries in the range of 78.8-93.8% with relative standard deviations below 6.9%. The limits of quantitation for the three compounds were below 0.183 mu g/kg. The proposed method was validated by analyzing real samples.