Improvement in bioactlve protein storage stability and colon-targeted release:a simple double-layer chitosan-based particle

摘要

Aim:To improve the long-term storage stability of a bioactive protein and the subsequent colon-targeted release,a double-layer chitosan(CS)-based particle was developed.Methods:To form the double-layer CS-based particle,the second layer was crosslinked onto the single-layer bovine serum albumin(BSA)-loaded CS-based particle.The structure and properties of particles were further investigated.Results:With the second layer,the double-layer particles became more compact,which was important for the inhibition of bioactive protein leakage during storage through strong electrostatic interactions and swelling of the hydrogels.After 30 d of storage,there was only 43.74-49.32% BSA leakage from the C15-TPP/C15-HMP double-layer particles.Moreover,the BSA release in subsequent colon-targeted delivery after storage was 44.02-48.59%.Conclusions:With double-layer shielding and a more compact arrangement,it was possible to reduce bioactive protein leakage over long periods storage and achieve subsequent colon-targeted delivery.