Molecular Linkage Map for the Silkworm, Bombyx mori, Based on Restriction Fragment Length Polymorphism of cDNA Clones

摘要

A cDNA linkage map in the domesticated silkworm, Bombyx mori, based entirely on backcross segregant analysis is reported. One hundred segregants from a single pair mating derived from a backcross of a RF02 female and a F_1 (RF02 x RF50) male were usedas the mapping population. More than 194 randomly isolated cDNA clones whose linkage groups had been determined previously were analyzed out of which 189 were unambiguously placed on the linkage map by repeated three-point analysis. Allelic differencesin cDNA markers were based on RFLP analysis by Southern blot hybridization. Every recombinant value for each clone on the same chromosome was calculated directly. The majority of the mapped clones corresponded to single loci dispersed on every 28 chromosomes. The map covers about 66% of the genome and provides important landmarks for Bombyx genome analysis