Identification and pathogenicity of plant viruses infecting Valeriana fauriei in Japan

摘要

Samples from Valeriana fauriei Briq. plants were collected from six prefectures to survey viral pathogens. By next-generation sequencing and RT-PCR analysis, asparagus virus 2 (AV-2), broad bean wilt virus 2 (BBWV-2), cucumber mosaic virus (CMV), gaillardia latent virus (GaILV), ligustrum necrotic ringspot virus (LNRSV), and tomato spotted wilt orthotospovirus (TSWV) were detected. Many collected samples were coinfected with 2-4 viruses. V. officinalis was also back-inoculated with one or a mixture of isolated viruses to confirm pathogenicity of isolated viruses. This report is the first of GaILV and LNRSV in Japan and the first of AV-2 on V. fauriei.