Preparing Monoclonal Antibodies agains BYDV Using Viruses Purified from Natur Infected Plants

摘要

After a severe epidemic of barley yellow dwarf disease (BYD) on small grain crops that took place in European Russia in 1988-1991, the need for a BYDV identification method became evident. As we did not have diagnostic kits for BYDV detection, we could only identify the disease based on visual symptoms or by electronic microscopy of purified virus preparations and their UV spectra. To solve this problem, we prepared monoclonal antibodies (MAbs) against the viral preparations purified from naturally infected plants. This task had special features and difficulties: 1) viral yields were usually low, and 2) generally, the viral preparations consisted of a mixture of different BYDVs, sometimes polluted with other cereal viruses. In any case, we were able to separate BYDV through zonal centrifugation in sucrose gradient.