Elucidation of fungal dye-decolourizing peroxidase (DyP) and ligninolytic enzyme activities in decolourization and mineralization of azo dyes

摘要

Aim The aim of the study is to investigate the efficiency ofGeotrichum candidumin the decolourization and mineralization of synthetic azo dyes. Methods and Results It includes screening of enzymes fromG. candidumand its optimization, followed by decolourization and mineralization studies. Decolourization was observed to be maximum in methyl orange (94 center dot 6%) followed by Congo red (85%), trypan blue (70 center dot 4%) and Eriochrome Black T (55 center dot 6%) in 48 h, suggesting the plausible degradation of the azo dyes byG. candidum. The enzyme activity study showed that DyP-type peroxidase has highest activity of 900 mU ml(-1)compared to that of laccase (405 mU ml(-1)) and lignin peroxidase (LiP) (324 mU ml(-1)) at optimized pH (6) and temperature (35 degrees C). Moreover, the rate of decolourization was found to be directly proportional to the production of laccase and LiP, unlike DyP-type peroxidase. Furthermore, mineralization study demonstrated reduction in aromatic amines, showing 20% mineralization of methyl orange. Conclusion Geotrichum candidumwith its enzyme system is able to efficiently decolourize and mineralize the experimental azo dyes. Significance and Impact of the Study The efficient decolourization and mineralization of azo dyes makesG. candiduma promising alternative in the treatment of textile effluent contaminated with azo dyes.