METHOD FOR INCREASING ENZYME ACTIVITY OF HIGHLY RECOMBINANT DYE DECOLORIZING PEROXIDASE

摘要

Provided is a method for increasing the enzyme activity of a highly recombinant dye decolorizing peroxidase, related to the technical field of bioengineering. Comprised are methods (1) and/or (2). For (1), a dye decolorizing peroxidase (DyP) is heterologously expressed in Escherichia coli, a recombinant strain is acquired and fermented, by adding an exogenous substance glutamate and/or FeCl₂ to a fermentation culture medium, when 80 μmol/L of FeCl₂ is added to the culture medium, the enzyme activity is increased to 1.45 times of that of a control. For (2), by means of the co-expression of hemA genes and DyP genes of a heme synthesis pathway key enzyme, combined with exogenously added 40 μmol/L glutamate, the enzyme activity of the DyP is increased to 13.2 U/mg, and dye decolorization rate is increased to 53.3%.