Development and validation of modified QuEChERS method coupled with LC–MS/MS for simultaneous determination of cymiazole, fipronil, coumaphos, fluvalinate, amitraz, and its metabolite in various types of honey and royal jelly

摘要

Highlights?Six pesticide residues in four types of honey and royal jelly were determined by LC–MS/MS analysis.?Samples were extracted by a modified EN-QuEChERS method using both C18 sorbent and PSA.?Recovery was good when the honey samples were extracted with 0.2M dibasic sodium phosphate buffer and acetonitrile.?Three of the market samples tested positive for cymiazole residues.AbstractOver the past few decades, honey products have been polluted by different contaminants, such as pesticides, which are widely applied in agriculture. In this work, a modified EN – quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction method was developed for the simultaneous quantification of pesticide residues, including cymiazole, fipronil, coumaphos, fluvalinate, amitraz, and its metabolite 2,4-dimethylaniline (2,4-DMA), in four types of honey (acacia, wild, chestnut, and manuka) and royal jelly. S