A fast, sensitive, and high throughput method for the determination of esomeprazole in dog plasma by UHPLC–MS/MS: Application to formulation development of the compound preparation of esomeprazole

摘要

Highlights?Developing and fully validating the UHPLC–MS/MS method for the analysis of esomeprazole.?Evaluating pharmacokinetic parameters of esomeprazole in dog plasma.?Optimizing the prescription of modified esomeprazole with sodium bicarbonate.?Researching the pharmacokinetic study three doses of pharmaceuticals in dogs.?Provide a promising approach to improve the stabilization of acid-labile esomeprazole.AbstractTo investigate deeply into the preclinical pharmacokinetics and prescription design of esomeprazole, a sensitive, high throughput and robust UHPLC–MS/MS method had been developed and fully validated for the analysis of esomeprazole in dog plasma. Esomeprazole and diazepam (IS) were fast extracted from plasma by alkalified organic solvent, and separated on MP-C18