Sensitive Method for Measuring Cis-Diol Containing Compounds in Plasma Using 2D-LC-MS/MS

摘要

The present invention provides methods for determining the amount of cis-diol containing compounds in a biological samples using mass spectrometry, particularly catecholamines (dopamine, epinephrine, norepinephrine) in a plasma sample. After a sample is mixed with an internal standard, extracted with a two-step liquid-liquid extraction process utilizing phenylboronic acid chemistry, and then derivatized with a benzoyl chloride-like agent, relevant moieties are then separated chromatographically and detected by MS/MS whereby specific ion transitions are monitored. This invention has several clinical utilities, particularly those related to measuring plasma catecholamines in patients.