Analysis of Major Components of Bacitracin, Colistin and Virginiamycin in Feed Using Matrix Solid-phase Dispersion Extraction by Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry

摘要

A quantitative LC-MS/MS method has been developed for simultaneous determination of bacitracin A, bacitracin B, colistin A, colistin B and virginiamycin M-1 in feed. This rapid simple and effective extraction method was based on matrix solid-phase dispersion. Qualitative and quantitative analyses were performed by LC-ESI-MS/MS. CC beta of polypeptide antibiotics upon the method ranged from 9.6 to 15.8 mu g kg(-1) and 19.4 to 27.5 mu g kg(-1), respectively. The limit of quantification of polypeptide antibiotics was 25 mu g kg(-1) in feed samples. The recoveries of polypeptide antibiotics spiked in feed samples at a concentration range of 25-100 mu g kg(-1) were found above 75.9-87.9% with relative standard deviations within days less than 15.7% and between days less than 20.6%. This rapid and reliable method can be used to efficiently separate, characterize and quantify the residues of polypeptide antibiotics in feed with advantages of simple pretreatment and environmental friendly.