Bone substitute materials supplemented with prolyl hydroxylase inhibitors decrease osteoclastogenesis in vitro

摘要

Background and objective: Inhibition of prolyl hydroxylases stimulates bone regeneration. Consequently, bone substitute materials were developed that release prolyl hydroxylase inhibitors. However, the impact of prolyl hydroxylase inhibitors released from these carriers on osteoclastogenesis is not clear. We therefore assessed the effect of bone substitute materials that release prolyl hydroxylase inhibitors on osteoclastogenesis. Material and methods: Dimethyloxa-lylglycine, desferrioxamine, and L-mimosine were lyophilized onto bovine bone mineral and hydroxyapatite, and superna-tants were generated. Osteoclastogenesis was induced in murine bone marrow cultures in the presence of the superna-tants from bone substitute materials. The formation of tartrate-resistant acid phosphatase (TRAP)-positive multi-nucleated cells and TRAP activity were determined. To test for possible effects on osteoclast progenitor cells, we measured the effect of the supernatants on proliferation and viability.