Precipitation of Lysozyme Nanoparticles from Dimethyl Sulfoxide Using Carbon Dioxide as Antisolvent

摘要

The protein lysozyme has been precipitated as amorphous nanoparticles from a DMSO solution usimg dense carbon dixide as antisolvent by aplying the so-called gas sntisolvent recrystallization technique in a 400-mL precipitator The objectife is to investigate4 the possibility of tuning the particle properties by changing the key process parameters.namely antisolvnt addition rate initial solute concentration and temperature.It is shown that none of these operating praameters has a major effect on the average particle size or the particle sinz distribution.The formoer is mostal between 200 and 300 nm and exhibits no evident greds The latter is always unimodal and rather narrow and exhibits increasing agglomeration at higher tempersture and initial solute comcental results compare well with date data about the same sytem obtained present experimental results compare well sith date agout the same system obtained in a different edxperimentasl setup which were previously reported in literature,thus pointing at the reproducibility and robusteness of GAS antiaslvent recrystallization.Moreover these are consistent with the theoretical understanding of gas antisolvent recrystallaztion as fachieved by using a recently developed model of the process.