Application of Waste Activated Bleaching Earth Containing Rapeseed Oil on Riboflavin Production in the Culture of Ashbya gossypii

摘要

Waste activated bleaching earth (ABE) that contained 40% rapeseed oil and was discharged by an oil refinery plant,was used for riboflavin production in a culture of Ashbya gossypii.When 125 g/L waste ABE that contained 50 g/L rapeseed oil was added into the culture,the riboflavin concentration was 1.12g/L which was almost 1.6-fold as high as that of pure repeseed oil.However,in waste ABE concentration higher than 125 g/L the produced riboflavin concentration decreased,which was due to the difficulty on mixing due to the presence of a high amoutn of solid material in the culture.The surface of the waste ABE was smooth without a hithch,because of being covered with rapeseed oil.However,after the culture the surface of the waste ABE seemed like that of new one and the oil content was nearly zero grams per liter The waste ABE oily caly and its black color gradually fade and yellow little by little and finally the waste ABE changed to yellow powder.Of the ribofavin produced during the culture 70% was assorbed in the oil free wate ABE With diluted alkali soution extraction only two times yieled 90% recovery of riboflavin adsorbed in the waste ABE The waste ABE containing waste vegtable oil was suitable for raw material for production of hte value-added useful bioproucts,which night be a good model.for reuse of the waste resoure.