Determination of the Solvent Blue 35 dye in diesel fuel by solid phase extraction and high-performance liquid chromatography with ultraviolet detection

摘要

The method for the determination of blue dye Solvent Blue 35 (SB 35) in diesel fuel by high performance liquid chromatography (HPLC) coupled with UV-VIS detection was developed. The dye is separated from the hydrocarbon sample matrix and determined as a single component at 650 nm wavelength. Solid phase extraction (SPE) on the silica based solid phase sorbent was used as a pre-treatment method. Among investigated elution solvent mixtures, the mixture composed of chloroform and heptane in the volume ration of 1:0.75 (v/v) provided the best SB 35 recovery. During method validation two calibration models were tested: external calibration and matrix-matched calibration. For both models correlation coefficients were higher than 0.99 and slopes were compared using the Student's t-test. Precision was evaluated as repeatability and intermediate precision with relative standard deviations less than 7%. Low limit of quantification of 0.1 mg L-1 were obtained. Optimized procedure using 250 mg of sorbens provided recoveries ranged from 76.9% to 99.4% over a linear range (0.1-10 mg L-1). The developed method has been applied to the analysis of blue diesel samples from the market and suspicious samples. The content of SB 35 dye in a commercial blue diesel samples was found to be around 0.5 mg L-1 while presence of SB 35 dye in suspicious sample was confirmed.