The A30P alpha-synuclein mutation decreases subventricular zone proliferation

摘要

Parkinson's disease (PD) is associated with olfactory defects in addition to dopaminergic degeneration. Dopaminergic signalling is necessary for subventricular zone (SVZ) proliferation and olfactory bulb (OB) neurogenesis. Alpha-synuclein (alpha-syn or Snca) modulates dopaminergic neurotransmission, and SNCA mutations cause familial PD, but how alpha-syn and its mutations affect adult neurogenesis is unclear. To address this, we studied a bacterial artificial chromosome transgenic mouse expressing the A30P SNCA familial PD point mutation on an Snca(-/-) background. We confirmed that the SNCA-A30P transgene recapitulates endogenous alpha-syn expression patterns and levels by immunohistochemical detection of endogenous alpha-syn in a wild-type mouse and transgenic SNCA-A30P alpha-syn protein in the forebrain. The number of SVZ stem cells (BrdU+GFAP+) was decreased in SNCA-A30P mice, whereas proliferating (phospho-histone 3+) cells were decreased in Snca(-/-) and even more so in SNCA-A30P mice. Similarly, SNCA-A30P mice had fewer Mash1+ transit-amplifying SVZ progenitor cells but Snca(-/-) mice did not. These data suggest the A30P mutation aggravates the effect of Snca loss in the SVZ. Interestingly, calbindin+ and calretinin (CalR)+ periglomerular neurons were decreased in both Snca(-/-), and SNCA-A30P mice but tyrosine hydroxylase+ periglomerular OB neurons were only decreased in Snca(-/-) mice. Cell death decreased in the OB granule layer of Snca(-/-) and SNCA-A30P mice. In the same region, CalR+ numbers increased in Snca(-/-) and SNCA-A30P mice. Thus, alpha-syn loss and human A30P SNCA decrease SVZ proliferation, cell death in the OB and differentially alter interneuron numbers. Similar disruptions in human neurogenesis may contribute to the olfactory deficits, which are observed in PD.