A Solution for Manufacturing Antibody Medicines

摘要

Antibody medicines are manufactured by using genetically modified animal cells. The major nutrient of cells is glucose, and so maintaining an appropriate concentration of glucose for the number and condition of cells in the culture is an effective way to increase the production of antibodies and ensure their stable quality. To improve this control, we have developed a bioreactor system consisting of three devices: (1) a near-infrared spectroscopy (IWIRS) inline sensor for measuring glucose and lactate concentration, both of which are indicators of cell metabolism, (2) an impedance spectroscopy inline sensor for measuring the number of viable cells, and (3) a controller that predicts the volume of glucose consumption based on cell metabolism and automatically feeds glucose solution. We carried out an experiment with a prototype of this system and verified that it maintained glucose concentration within a set range throughout the culture period, hi this paper, we describe element technologies implemented in this system and report the results of the experiment.