Estimation of glomerular filtration rate in normal sheep by the disappearance of iohexol from serum.

摘要

15 healthy sheep, 10 ewes and 5 rams (6 Dala, 9 Spaelsau) of 6 to 12 months of age, were tested for clearance of iohexol in serum. An indwelling catheter was placed in the jugular vein and 600 mg I/kg body weight of iohexol (Omnipaque, 300 mg iodine/ml) was injected. Blood samples were taken from the jugular vein on the opposite side immediately before injection and at various times up to 300 min after injection. The procedure was performed twice on all animals with 14 to 21 days between first andsecond trials. There were no adverse effects after iohexol injections and all sheep were healthy during, and for a month after the study. The mean clearance of iohexol estimated by a two-compartment method was 1.8 ml/min per kg (95% confidence limits, 1.6 to 2.0 ml/min per kg) in the first trial and 1.7 ml/min per kg (1.5 to 1.9 ml/min per kg) in the second trial. The mean glomerular filtration rate (GFR) for iohexol estimated by a one-compartment method was 1.9 ml/min per kg (1.7 to 2.2 ml/min per kg)in the first trial and 1.8 ml/min per kg (1.6 to 2.0 ml/min per kg) in the second. The GFR values were similar to those reported for the inulin method. It is concluded the iohexol can be used for estimating GFR in sheep, and it is easier to use than theinulin.