Production, Purification and Enzyme Activity of Lipase from Aspergillus oryzae and Trichoderma viride

摘要

Lipases are placed after proteases and carbohydrases in world enzyme market and share about 5% of enzyme market. They occur in plants, animals and microorganisms and are accordingly classified as plant, animal and microbial lipases. Wherever they exist, they function to catalyze hydrolysis of triglycerides to glycerol and fatty acid. Fungi are the potent source for the lipase production. The pure culture of Aspergillus oryzae and Trichoderma viride shows maximum lipase production in 96 and 72-96 hours respectively. Lipase Assay was done by using para Nitrophenyl Palmitate (pNPP). The lipase activity was determined from the crude enzyme as well as 35%, 60% and from dialyzed sample of lipase. Enzyme activity, protein content as well as total lipase activity was observed more in case of Aspergillus oryzae rather than Trichoderma viride.