Secondary metabolites produced by an endophytic fungus Cordyceps ninchukispora from the seeds of Beilschmiedia erythrophloia Hayata

摘要

Graphical abstractDisplay OmittedHighlights?Four new amides were isolated fromCordyceps ninchukisporaBCRC 31900.?Thirteen known compounds were reported from this fungus for the first time.?The structures of the new compounds were determined by spectroscopic analysis.?Inhibitory effects of 10 compounds on NO production were evaluated.?Compound 3→4 reduced NO levels in LPS-stimulated RAW264.7 cells.Four new amide derivatives, designated as cordycepiamides A–D (1–4), together with 14 known compounds (5–18), were isolated from the EtOAc-soluble fraction of the 95% EtOH extract of long-grain rice fermented with the endophytic fungusC. ninchukisporaBCRC 31900, derived from the seeds of medicinal plantBeilschmiedia erythrophloiaHayata. Their structures were elucidated by means of spectroscopic and mass-spectrometric analyses, particularly 1D and 2D NMR spectroscopy as well as HRESIMS. All known isolates except11, were isolated for the first time from this species. The antiinflammatory activities of selected isolated 10 compounds (1, 2, 4–6,9–12, and14) were evaluated as inhibitory activities against lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW264.7 cell lines. Compound3→4was shown to have modest anti-inflammatory effects through inhibition of NO production in LPS-stimulated RAW264.7 cells.