MicroRNA-494 promotes the proliferation and migration of human glioma cancer cells through the protein kinase B/mechanistic target of rapamycin pathway by phosphatase and tensin homolog expression

摘要

The aim of the present study was to analyze the possible association between microRNA-494 (miR-494) and cell proliferation in glioma cancer. Firstly, the expression of miR-494 was revealed to be upregulated in patients with glioma, compared with the normal group. Next, anti-miR-494 mimics were used to decrease the expression of miR-494 in glioma cancer cells, which subsequently induced apoptosis, and inhibited cell growth and migration. Downregulation of miR-494 expression induced phosphatase and tensin homolog (PTEN) and suppressed the protein kinase B/mechanistic target of rapamycin pathway (Akt/mTOR) pathway in glioma cancer cells. By contrast, overexpression of miR-494 by miR-494 mimics promoted cell growth and migration, and suppressed the apoptosis of glioma cancer via the Akt/mTOR pathway by PTEN expression. Furthermore, a PTEN inhibitor was used to attenuate the function of miR-494 in glioma cancer autophagy through Akt/mTOR pathway. The promotion of PTEN promoted the function of anti-miR-494 on glioma cancer cell growth through Akt/mTOR pathway. Collectively, these results demonstrate that the effect of miRNA-494 on the proliferation and migration glioma cancer cells was mediated through Akt/mTOR pathway by PTEN expression.