LC-MS-based Screening of East Indian Sandalwood Oil for Mycobacterium tuberculosis Shikimate Kinase and Plasmodium falciparum Thioredoxin Reductase Inhibitory Activities

摘要

M tuberculosis shikimate kinase (MtSK) and P. falciparum thioredoxin reductase (PfTrxR) represent promising drug targets in tuberculosis and malaria therapy respectively. East Indian Sandalwood oil (EISO) has many reported therapeutic benefits including antibacterial and antiplasmodial effects. The aim of this study was to screen EISO for MtSK and PfTrxR inhibition as potential mechanisms of antimycobacterial and antiplasmodial activities. Liquid chromatography-mass spectrometry (LC-MS) functional assays were used to assess MtSK and PfTrxR inhibition. In this study, EISO displayed antitubercular activity against M tuberculosis H37Rv at MIC value of 0.002% v/v. Subsequently preliminary screening of the MtSK inhibition by EISO showed an IC50 value of 0.015% v/v. Additionally, EISO presented 90% inhibition of PffrxR at 0.01% v/v suggesting a potential mechanism of action for reported antimalarial activity.