Advances in nanomaterial based optica! biosensing and bioimaging of apoptosis via caspase-3 activity: a review

摘要

Caspase-3 plays a vital role in intrinsic and extrinsic pathways of programed cell death and in cell proliferation.Its detection is an important tool for early detection of some cancers and apoptosis-related diseases,and for monitoring the efficacy of pharmaceuticals and of chemo-and radiotherapy of cancers.This review(with 72 references)summarizes nanomaterial based methods for signal amplification in optical methods for the determination of caspase-3 activity.Following an introduction into the field,a first large section covers optical assays,with subsections on luminescent and chemiluminescence,fluorometric(including FRET based),and colorimetric assays.Further section summarize methods for bioimaging of caspase-3.A concluding section covers current challenges and future perspectives.