Isolation and Identification of Metabolites in Chinese Northeast Potato (Solanum tuberosum L.) Tubers Using Gas Chromatography-Mass Spectrometry

摘要

The gas chromatography coupled with mass spectrometry was established for the isolation and identification of the 65 compounds and main compounds including sugar, sugar alcohols, dimeric and trimeric saccharides, amino acid, and organic acid, in Chinese northeast potato (Solanum tuberosum L.) tubers. Polar metabolites were extracted and separated from potato tubers of the four kinds of potatoes in Chinese northeast with 80% methanol, and derived with N, O-Bis (trimethylsilyl) trifluoroacetamide. Then, the analytical performances of the proposed method were evaluated. When the reproducibility was evaluated, the relative standard deviations (RSDs) of both peak count of 90.4% metabolites and peak areas of 91.4% metabolites were lower than 30%. When the stability was evaluated, the RSDs of both peak count of 76.7% metabolites and cumulative peak areas of 92.5% metabolites were lower than 30%. In contrast to the databases, some of the primary and secondary metabolites were identified. The established method is expected to be useful for the metabolomic studies of potato.