Association of RAPH1 expression with cervical cancer progression and aggressiveness

摘要

Objective: The gene known as Ras association (RalGDS/AF-6) and pleckstrin homology domains I (RAPH1) play a role in cellular motility and lamelipodial protrusion, raising the possibility that it may contribute to development of cervical carcinoma and aggressive invasion by tumor cells. Materials and Methods: RAPH1 expression was analyzed in 153 cervical tissue samples at the mRNA level using quantitative RT-PCR and at the protein level using immunohistochemistry. Correlations were explored between expression and clinicopathological features of tumors. Results: Levels of RAPH1 mRNA were significantly higher in carcinoma tissues than in precancerous squamous intraepithelial lesions (SIL) or normal tissue (both p 0.05). The percentage of RAPH1-positive samples increased progressively from normal tissue (10.0%) and low-grade SIL (31.3%) to high-grade SIL (45.8%) and carcinoma tissue (72.3%; (chi 2CMH) = 30.0, p 0.001). RAHP1 expression positively correlated with grade of tumor differentiation (p = 0.04), vascular invasion (p 0.001), interstitial invasive depth (p 0.01), and tumor size (p 0.01). Conclusions: RAPH1 expression is associated with cervical cancer development and aggressive behavior.