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A Novel Photo‐Induced Electrochemical Biosensing Method Based on Fluorescent Labeled Molecular Beacon

机译：一种基于荧光标记分子标记的新型光诱导的电化学生物传感方法

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Abstract >A novel photo‐induced electrochemical biosensing method has been developed based on fluorescence quenching effect and electrochemical method. In this sensing strategy, the molecular beacon probes labeled with methylene blue were immobilized on the gold nanoparticles modified gold electrode surface firstly; then dopamine was assembled on the electrode surface through electrostatic interaction with gold nanoparticles. Under the continuous illumination, the fluorescence of the methylene blue was quenched by the gold nanoparticles before hybridization; after hybridization with the complementary DNA, methylene blue was far away from the gold nanoparticles and the fluorescence recovered, and then singlet oxygen was generated in the photosensitive reaction of methylene blue in the presence of dissolved oxygen. Singlet oxygen reacted with dopamine, which resulted in the reduction of concentration of the dopamine on the electrode surface. The current of the dopamine on the electrode was used for the sensing of the conformational change of molecular beacon and hence for the detection of target DNA. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >一部小说光诱导的电化学生物传感方法基于荧光猝灭效应和电化学方法开发。在这种感测策略中，将用亚甲基蓝色标记的分子信标探针首先固定在金纳米颗粒上的金电极表面上;然后通过与金纳米颗粒的静电相互作用在电极表面上组装多巴胺。在连续照明下，在杂交前通过金纳米颗粒淬灭亚甲基蓝的荧光;在用互补DNA杂交之后，亚甲基蓝离金纳米颗粒远离金纳米颗粒，回收荧光，然后在溶解氧存在下在亚甲蓝的光敏反应中产生单次氧。单向氧与多巴胺反应，导致电极表面上的多巴胺的浓度降低。电极上的多巴胺的电流用于感测分子信标的构象变化，从而检测靶DNA。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22546/'>《Electroanalysis》</a> <b style="margin: 0 2px;">|</b><span>2017年第5期</span><b style="margin: 0 2px;">|</b><span>共6页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Panpan&option=202" target="_blank" rel="nofollow">Zhang Panpan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Arken Gulijamal&option=202" target="_blank" rel="nofollow">Arken Gulijamal;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Guixin&option=202" target="_blank" rel="nofollow">Li Guixin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guan Ming&option=202" target="_blank" rel="nofollow">Guan Ming;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tian Shuge&option=202" target="_blank" rel="nofollow">Tian Shuge;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Engineering Research Center of Electrochemical Technology and Application School of Chemistry and Chemical EngineeringXinjiang Normal UniversityUrumqi Xinjiang 830054 P.R. China;</p> <p>Engineering Research Center of Electrochemical Technology and Application School of Chemistry and Chemical EngineeringXinjiang Normal UniversityUrumqi Xinjiang 830054 P.R. China;</p> <p>Engineering Research Center of Electrochemical Technology and Application School of Chemistry and Chemical EngineeringXinjiang Normal UniversityUrumqi Xinjiang 830054 P.R. China;</p> <p>Engineering Research Center of Electrochemical Technology and Application School of Chemistry and Chemical EngineeringXinjiang Normal UniversityUrumqi Xinjiang 830054 P.R. China;</p> <p>Central Laboratory of Xinjiang Medical UniversityUrumqi Xinjiang 830011 P.R.China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4817.html" title="电化学、电解、磁化学">电化学、电解、磁化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Photo-induced electrochemical biosensing method&option=203" rel="nofollow">Photo-induced electrochemical biosensing method;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Molecular beacon&option=203" rel="nofollow">Molecular beacon;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Singlet oxygen&option=203" rel="nofollow">Singlet oxygen;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dopamine&option=203" rel="nofollow">Dopamine;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Photosensitive reaction&option=203" rel="nofollow">Photosensitive reaction;</a> </p> <div class="translation"> 机译：光学诱导的电化学生物传感方法;分子信标;单次氧;多巴胺;光敏反应; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" 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